Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: Bioactive Materials

Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

doi: 10.1016/j.bioactmat.2025.12.055

Figure Lengend Snippet: In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

Techniques: In Vitro, Cell Culture, Staining, Marker, Fluorescence, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

Journal: Bioactive Materials

Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

doi: 10.1016/j.bioactmat.2025.12.055

Figure Lengend Snippet: In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

Techniques: In Vivo, Immunohistochemistry, Staining

Journal: iScience

Article Title: Circadian disruption exacerbates MASH by reducing Akkermansia muciniphila via the FXR-CYP7A1-bile acid axis

doi: 10.1016/j.isci.2026.115397

Figure Lengend Snippet: Circadian disruption promotes inflammation, lipid accumulation, and fibrosis in MASH mice (A) The relative mRNA expression of lipid-metabolism-related genes ( Srebp , Fasn , and Acaca ), inflammation-related genes ( Cxcl2 , Ccl2 , and Nos2 ), and fibrosis-related genes ( Col3a1 , Col4a1 , and α-Sma ) in MASH with or without circadian disruption, n = 6. (B) Flow cytometry analysis of the abundance of macrophages (CD11b + F4/80 + ), M1 cells (CD86 + CD206 − ), and M2 cells (CD86 − CD206 + ) in the liver in each group, n = 6. (C) Comparison of M1/M2 polarization in the liver in each group by CD86/CD206 detected by immunohistochemistry. Scale bars: 100 μm (10×). (D) Serum IL-6, IL-1β, and TNF-α in each group, n = 6. (E) The relative expression level of the oxidative stress indicator ROS, the content of GSH, and the relative mRNA expression level of Hif-1α related to them in each group, n = 6. (F) The total number of genes detected by liver transcriptomics, as well as the number of genes that are relatively upregulated and downregulated in the two groups. (G) NMDS plot of the main components of liver transcriptomics in each group, n = 4. (H) Reactome analysis results (barplot) of liver transcriptomics in the two groups. (I) Reactome analysis results (dotplot) of liver transcriptomics in the two groups. Data are expressed as mean ± SD ( n = 4–6), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, compared with the MASH group. p values were calculated using unpaired t test. Srebp , sterol regulatory element-binding protein; Fasn , fatty acid synthase; Acaca , acetyl-CoA carboxylase; Cxcl2 , chemokine (C–X–C motif) ligand 2; Ccl2 , chemokine (C–C motif) ligand 2; Nos2 , nitric oxide synthase 2; Col3a1 , collagen alpha-1 (III) chain; Col4a1 , collagen alpha-1 (IV) chain; α-Sma , α-smooth muscle actin; M1, M1 polarization of macrophages; M2, M2 polarization of macrophages; CD11b, marker of mouse macrophages; F4/80, marker of mouse macrophages; CD86, marker of M1 polarization of mouse macrophages; CD206, marker of M2 polarization of mouse macrophages; IL-6, interleukin-6; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor-alpha; ROS, reactive oxygen species; GSH, glutathione; Hif-1α , hypoxia inducible factor-1 alpha; NMDS, non-metric multidimensional scaling.

Article Snippet: Rabbit anti-CD86 antibody , Cell Signaling Technology , Cat# 91882; RRID: AB_2797422.

Techniques: Disruption, Expressing, Flow Cytometry, Comparison, Immunohistochemistry, Transcriptomics, Binding Assay, Marker